Question : Local Printing Broken only for Wyse Clients, only on one Citrix server

This one is driving me crazy:
This began after updating my Citrix 4.5 farm with the  PSE450W2K3R03 updates.
When Wyse clients connect to CitrixServer01 all printers map correctly, however when they attempt to print from M$oft word, the print dialog box appears, but does not respond if you click "OK" or "Properties".
I am able to print test pages correctly.
This ONLY affects Wyse thin clients attached to this server.
Fat clients are not affected.
The same Wyse stations work correctly if attached to a different server.
????

Answer : Local Printing Broken only for Wyse Clients, only on one Citrix server

I would take a look at at  post hfr03 hotfix PSE450R03W2K3016 - http://support.citrix.com/article/CTX119086:

Autocreated printers might fail to fully initialize in the session, causing a "The printer is not installed" error message to appear.

While you may or may not be getting the error message, it sounds like your issue - your printers don't seem to be auto creating correctly.

Another step to try assuming you have roaming user profiles - try rebuilding a test user's tsprofile and let it recreate at next login and see if that fixes it.