I assume you are looking into this because your existing servers are falling out of warranty, right?
Because for 50 users, unless they were falling out of warranty, I don't know why you are replacing them. For 50 users, the hardware is generally excessive.
If you are looking to consolidate, then I'd suggest two physical servers with one running Hyper-V should be enough... but you should also look into getting EBS - essential business server.